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KMID : 1123920100240010111
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Korean Journal of Oriental Physiology and Pathology
2010 Volume.24 No. 1 p.111 ~ p.117
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Effect of Garlic Extracts with Extraction Conditions on Antioxidant and Anticancer Activity
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Kim Hae-Ja
Han Choong-Ho
Kim Nan-Yeong
Lee Eun-Kyoung
Lee Ki-Nam
Cho Hwa-Eun
Choi Yun-hee
Chong Myongsoo
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Abstract
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The purpose of this study was to investigate the effect of garlic(Allium sativum L.) extracts with extraction conditions on antioxidant and anticancer activity. The extracts prepared for garlic by hot temperature extraction (HG), low temperature extraction (LG), UMPM extraction (UG), fermentation (FG) and black garlic hot temperature (BG) method. Content of total polyphenol compound was the BG higher than other extracts. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manners, and the activity of BG and UM was significantly higher than LG and FG. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. All extracts does not have any toxic effects in macrophages(Raw 264.7). And UG inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of LG and UG on HT 1080 cell was indicated a significantly inhibition activity. These results suggested that UG (UMPM extraction of garlic) have activities of antioxidant, anticancer effects.
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KEYWORD
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garlic, allium sativum L., antioxidant, anticancer, Raw 264.7 cell, HT 1080 cell
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